Proceedings of the Komi Science Centre of the Ural Division of the Russian Academy of Sciences Proceedings of the Komi Science Centre of the Ural Division of the Russian Academy of Sciences Известия Коми научного центра УрО РАН 1994-5655 6a84bc5194fbba14f78674d0 10.19110/1994-5655-2023-6-21-24 Научные статьи Science articles Научные статьи Modeling a new form of oil-oxidizing preparations in the form of cell agglomerates of mixed cultures of microorganisms stabilized by polyelectrolytes and salts of higher fatty acids Разработка новой формы нефтеокисляющих препаратов в виде агломератов клеток смешанных культур микроорганизмов, стабилизированных полиэлектролитами и солями жирных кислот Тарабукин Д. В. Tarabukin D. V. dim1822@yandex.ru Щемелинина Т. Н. Schemelinina T. N. tatyanakomi@mail.ru Анчугова Е. М. Anchugova E. M. Маркарова М. Ю. Markarova M. Yu. Институт биологии ФИЦ Коми НЦ УрО РАН Сыктывкар Россия Institute of Biology, Federal Research Center Komi Science Center of the Ural Branch of the Russian Academy of Sciences Syktyvkar Russian Federation Институт биологии ФИЦ Коми НЦ УрО РАН Сыктывкар Россия Institute of Biology, Federal Research Center Komi Science Center of the Ural Branch of the Russian Academy of Sciences Syktyvkar Russian Federation Институт биологии ФИЦ Коми НЦ УрО РАН Сыктывкар Россия Institute of Biology, Federal Research Center Komi Science Center of the Ural Branch of the Russian Academy of Sciences Syktyvkar Russian Federation Институт биологии ФИЦ Коми НЦ УрО РАН Сыктывкар Россия Institute of Biology, Federal Research Center Komi Science Center of the Ural Branch of the Russian Academy of Sciences Syktyvkar Russian Federation 25 10 2023 25 10 2023 21 24 06 03 2023 07 07 2023 © 2023 Тарабукин Д.В., Щемелинина Т.Н., Анчугова Е.М., Маркарова М.Ю. © 2023 Tarabukin D.V., Schemelinina T.N., Anchugova E.M., Markarova M.Y. 2023 Тарабукин Д. В., Щемелинина Т. Н., Анчугова Е. М., Маркарова М. Ю. Tarabukin D. V., Schemelinina T. N., Anchugova E. M., Markarova M. Yu. https://ras.editorum.ru/en/nauka/publications/6a84bc5194fbba14f78674d0/view

Проведена оценка эффективности применения катионного полиакриламида и его гидрофобного производного на стадии отделения биомассы клеток от культуральной жидкости. Клетки микроорганизмов-нефтедеструкторов, полученных из смешанной культуры с помощью флокуляции и флотации, сохраняют жизнеспособность для дальнейшего использования при обработке нефтезагрязненных почв в нестерильных условиях. Применение новой формы нефтеокисляющего биопрепарата приводило к снижению содержания нефтепродуктов в загрязненной почве на 40 %.

he efficiency of application of cationic polyacrylamide and its hydrophobized derivative at the stage of cell biomass separation from the cell cultures fluid is estimated. Cells of oil-degrading microorganisms obtained from mixed culture fluid by flocculation and flotation retain their viability for further use in the treatment of oil-contaminated soils under non-sterile conditions. The application of a new form of oil-oxidizing preparation resulted in a 40 % reduction of total petroleum hydrocarbon content in the contaminated soil.

 нефтеокисляющие микроорганизмы нефтяное загрязнение флокуляции полиэлектролиты hydrocarbon-oxidizing microorganisms oil pollution flocculation polyelectrolytes This work was financially supported by 122040600019-1.

Hassavfnshahian, M. Isolation and characterization of two crude oil-degrading yeast strains, Yarrowia lipolytica PG-20 and PG-32, from the Persian Gulf / M. Hassanshahian, H. Tebyanian, S. Cappello // Marine Pollution Bulletin. - 2012. - № 64. - P. 1386-1391. Hassavfnshahian, M. Isolation and characterization of two crude oil-degrading yeast strains, Yarrowia lipolytica PG-20 and PG-32, from the Persian Gulf / M. Hassanshahian, H. Tebyanian, S. Cappello // Marine Pollution Bulletin. - 2012. - № 64. - P. 1386-1391. Biodegradation and detoxification of aliphatic and aromatic hydrocarbons by new yeast strains / M. Hashem, S. A. Alamri, S. A. A. Al-Zomyh, S. A. Alrumman // Ecotoxicology and Environmental Safety. - 2018. - № 151. - P. 28-34. Biodegradation and detoxification of aliphatic and aromatic hydrocarbons by new yeast strains / M. Hashem, S. A. Alamri, S. A. A. Al-Zomyh, S. A. Alrumman // Ecotoxicology and Environmental Safety. - 2018. - № 151. - P. 28-34. Ausheva, Kh. A. Development of a bacterial breparation based on immobilized cells / Kh. A. Ausheva, D. A. Goncharuk, E. S. Babusenko, S. A. Nekhaev, Z. S. Sultygova [et al.] // Theoretical Foundations of Chemical Engineering. - 2008. - № 5. - P. 767-773. Ausheva, Kh. A. Development of a bacterial breparation based on immobilized cells / Kh. A. Ausheva, D. A. Goncharuk, E. S. Babusenko, S. A. Nekhaev, Z. S. Sultygova [et al.] // Theoretical Foundations of Chemical Engineering. - 2008. - № 5. - P. 767-773. Onwurah, I. NE. Role of diazotrophic bacteria in the bioremediation of crude oil-polluted soil / I. NE. Onwurah // Journal of Chemical Technology and Biotechnology. - 1999. - Vol. 74, № 10. - P. 957-964. Onwurah, I. NE. Role of diazotrophic bacteria in the bioremediation of crude oil-polluted soil / I. NE. Onwurah // Journal of Chemical Technology and Biotechnology. - 1999. - Vol. 74, № 10. - P. 957-964. Mercier, M. Yeast suspension filtration: flux enhancement using an upward gas/liquid slug flow application to continuous alcoholic fermentation with cell recycle / M. Mercier, C. Maranges, C. Fonade, C. Lafforgue-Delorme // Biotechnology and Bioengineering. - 1998. - Vol. 58, № 1. - P. 47-57. Mercier, M. Yeast suspension filtration: flux enhancement using an upward gas/liquid slug flow application to continuous alcoholic fermentation with cell recycle / M. Mercier, C. Maranges, C. Fonade, C. Lafforgue-Delorme // Biotechnology and Bioengineering. - 1998. - Vol. 58, № 1. - P. 47-57. Lee, S. S. Microfiltration of recombinant yeast cells using a rotating disk dynamic filtration system / S. S. Lee, A. Burt, G. Rusotti, B. Buckland // Biotechnology and Bioengineering. - 1995. - Vol. 48, № 4. - P. 386-400. Lee, S. S. Microfiltration of recombinant yeast cells using a rotating disk dynamic filtration system / S. S. Lee, A. Burt, G. Rusotti, B. Buckland // Biotechnology and Bioengineering. - 1995. - Vol. 48, № 4. - P. 386-400. Perez-Torrado, R. Yeast biomass, an optimized product with myriad applications in the food industry / R. Perez-Torrado, E. Gamero, R. Gomez-Pastor, E. Garre, A. Aranda, E. Matallana // Trends in Food Science & Technology. - 2015. - № 46. - P. 167-175. Perez-Torrado, R. Yeast biomass, an optimized product with myriad applications in the food industry / R. Perez-Torrado, E. Gamero, R. Gomez-Pastor, E. Garre, A. Aranda, E. Matallana // Trends in Food Science & Technology. - 2015. - № 46. - P. 167-175. Berovic, M. A new method for the rapid separation of magnetized yeast in sparkling wine / M. Berovic, M. Berlot, S. Kralj, D. Makovec // Biochemical Engineering Journal. - 2014. - № 88. - P. 77-84. Berovic, M. A new method for the rapid separation of magnetized yeast in sparkling wine / M. Berovic, M. Berlot, S. Kralj, D. Makovec // Biochemical Engineering Journal. - 2014. - № 88. - P. 77-84. Tarabukin, D. V. Assessment of the lowland bog biomass for ex situ remediation of petroleum-contaminated soils / D. V. Tarabukin // Environments. - 2020. - Vol. 7 (86). - P. 1-11. - DOI: 10.3390/environments7100086. Tarabukin, D. V. Assessment of the lowland bog biomass for ex situ remediation of petroleum-contaminated soils / D. V. Tarabukin // Environments. - 2020. - Vol. 7 (86). - P. 1-11. - DOI: 10.3390/environments7100086. Moud, A. A. Polymer based flocculants: Review of water purification applications / A. A. Moud // Journal of Water Process Engineering. - 2022. - № 48 (7). - DOI : 10.1016/j.jwpe.2022.102938. Moud, A. A. Polymer based flocculants: Review of water purification applications / A. A. Moud // Journal of Water Process Engineering. - 2022. - № 48 (7). - DOI : 10.1016/j.jwpe.2022.102938. Satpute, S. K. Methods for investigating biosurfactants and bioemulsifiers: a review / S. K. Satpute, A. G. Banpurkar, P. K. Dhakephalkar, I. M. Banat, B. A. Chopade // Critical Reviews in Biotechnology. - 2010. - Vol. 30, № 2. - P. 127-144. Satpute, S. K. Methods for investigating biosurfactants and bioemulsifiers: a review / S. K. Satpute, A. G. Banpurkar, P. K. Dhakephalkar, I. M. Banat, B. A. Chopade // Critical Reviews in Biotechnology. - 2010. - Vol. 30, № 2. - P. 127-144. Varjani, S. J. Critical review on biosurfactant analysis, purification and characterization using rhamnolipid as a model biosurfactant / S. J. Varjani, V. N. Upasani // Bioresource Technology. - 2017. - № 232. - P. 389-397. Varjani, S. J. Critical review on biosurfactant analysis, purification and characterization using rhamnolipid as a model biosurfactant / S. J. Varjani, V. N. Upasani // Bioresource Technology. - 2017. - № 232. - P. 389-397.